Effects of Various Activators on Bovine Embryonic Development Following Intracytoplasmic Sperm Injection
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Abstract:
The objective of this study was to compare the effectiveness of various combination activators of bovine oocyte following intracytoplasmic sperm injection (ICSI) in terms of cleavage and embryo development. Bovine ICSI oocytes were treated with a variation of treatments of combined or single activators as follows; 30 V for 15 x 2 μsec of electrical pulse +10 μg/mL cycloheximide (CHX),7% ethanol + CHX, 5 μM Calcium ionophore A23187 (Ca-I)+ CHX, Ca-I + 1.9 mM 6-dimethylaminopurine (6-DMAP), CHX alone, Ca-I alone. The highest cleavage (73.5%-79.7%), and blastocyst rates (28.0%-35.3%) were obtained in Ca-I + 6-DMAP, ethanol + CHX and electrical stimuli + CHX groups. The treatment with CHX only or Ca-I only gave a significantly lower (P
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full textIntracytoplasmic Sperm Injection
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Journal title
volume 2 issue 2
pages 167- 173
publication date 2012-06-01
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